Reference gene standards for RT-qPCR – zebrafish

£85 per single assay or select any five assays for £375

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Gene symbol Accession number(s) Product code Amplicon length (bp) Intron: span/flank Intron length (bp) Amplicon %GC
actb NM_131031 dre_actb_001 148 span n/a 41.0
eef1a1a NM_200009 dre_eef1a1a_001
gapdh NM_001115114 dre_gapdh_001 85 span n/a 54.1
rpl13a NM_212784 dre_rpl13a_001 171 span n/a 51.0
tuba1 NM_194388 dre_tuba1_001 143 flank 1317 46.2


    ? Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
    ? No secondary structure in the amplicon for any assay (positive delta G)
    ? Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
    ? Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
    ? Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation


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