Reference gene standards for RT-qPCR – rodent

£85 per single assay or five assays for £375

To order please send a Purchase Order to finance@qstandard.co.uk

Free delivery within the UK

Rat Colour

 

Mouse

Gene symbol Accession number(s) Product code Amplicon length (bp) Intron: span/flank Intron length (bp) Amplicon %GC
Actb NM_007393 mmu_Actb_001 186 span n/a 55.7
B2m NM_009735 mmu_B2m_001 71 span n/a 53.5
Gapdh NM_008084 mmu_Gapdh_001 133 span n/a 53.4
Hmbs NM_013551 & NM_001110251 mmu_Hmbs_001 73 span n/a 49.3
Hprt1 NM_013556 mmu_Hprt_001 91 span n/a 62.6
Pla2g12a NM_023196 mmu_Pla2g12a_001 187 flank 11440 56.7
Rpl13 NM_023281 mmu_Rpl13_002 133 span 340 60.9
Sdha NM_016738 mmu_Sdha_001 85 no n/a 51.2
Tbp NM_013684 mmu_Tbp_001 232 span n/a 47.4

Rat

Gene symbol Accession number(s) Product code Amplicon length (bp) Intron: span/flank Intron length (bp) Amplicon %GC
Actb NM_031144 rno_Actb_001 300 flank 214 68.6
Gapdh NM_017008 rno_Gapdh_001 310 span n/a 52.6
Hmbs NM_013168.2 rno_Hmbs_001 151 span n/a 52.3
Hprt1 NM_012583 rno_Hprt1_002 115 span n/a 40.9
Rpl13 NM_031101 rno_Rpl13_001 76 span n/a 61.8
Sdha NM_130482 rno_Sdha_002 145 span n/a 49.0
Tbp NM_001004198 rno_Tbp_001 75 span n/a 41.3
Ywhaz NM_013011.3 rno_Ywhaz_001 148 span n/a 52.0

 

    ? Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
    ? No secondary structure in the amplicon for any assay (positive delta G)
    ? Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
    ? Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
    ? Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation

standard tubesno bg