| £85 per single assay or three assays for £225
To order please send a Purchase Order to finance@qstandard.co.uk Free delivery within the UK |
 |
Drosophila melanogaster
| Gene symbol | Accession number(s) | Product code | Amplicon length (bp) | Intron: span/flank | Intron length (bp) | Amplicon %GC |
|---|---|---|---|---|---|---|
| Act5c | NM_167053, NM_078497, NM_001014726, NM_001014725 | dme_Act5c_001 | 111 | no | n/a | 64.0 |
| eIF-4a | NM_164668, NM_164669, NM_057247, NM_164670 | dme_eIF-4a_001 | 165 | span | n/a | 55.6 |
| Gapdh2 | NM_080714, NM_080352 | dme_Gapdh2_001 | 70 | no | n/a | 48.6 |
| £85 per single assay or three assays for £225
To order please send a Purchase Order to finance@qstandard.co.uk Free delivery within the UK |
 |
Caenorhabditis elegans
| Gene symbol | Wormbase sequence name | Product code | Amplicon length (bp) | Intron: span/flank | Intron length (bp) | Amplicon %GC |
|---|---|---|---|---|---|---|
| cdc-42 | R07G3.1 | cel_cdc-42_001 | 129 | flank | 53 | 48.1 |
| pmp-3 | C54G10.3 | cel-pmp-3_001 | 73 | flank | 51 | 49.6 |
| Y45F10D.4 | Y45F10D.4 | cel_Y45F10D.4_001 | 83 | span | n/a | 50.0 |
- – Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
- – No secondary structure in the amplicon for any assay (positive delta G)
- – Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- – Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- – Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation
