Reference gene standards for RT-qPCR – human

£85 per single assay or select any five assays for £375

To order please send a Purchase Order to finance@qstandard.co.uk

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Gene symbol Accession number(s) Product code Amplicon length (bp) Intron: span/flank Intron length (bp) Amplicon %GC
ACTB NM_001101 hsa_ACTB_002 117 flank 110 55.6
ACTB NM_001101 hsa_ACTB_003 212 span n/a 68.6
ATP5E NM_006886 hsa_ATP5E_001 191 span n/a 42.4
B2M NM_004048 hsa_B2M_002 112 flank 3807 46.4
G6PD NM_021130 & NM_001042351 hsa_G6PD_002 80 flank 157 55.0
GAPDH NM_002046 hsa_GAPDH_001 87 span n/a 54.0
GNB2L1 NM_006098 hsa_GNB2L1_001 224 flank 2218 52.7
GPX1 NM_000581 hsa_GPX1_001 105 span n/a 53.3
GUSB NM_000181 hsa_GUSB_001 98 span n/a 48.0
HMBS NM_000190 & NM_001024382 hsa_HMBS_002 90 flank 1147 57.8
HPRT1 NM_000194 hsa_HPRT1_002 127 span n/a 43.3
PGK1 NM_000291 hsa_PGK1_001 176 span n/a 47.7
PLA2G6 NM_003560 hsa_PLA2G6_003 181 span n/a 59.6
POLR2A NM_003560 hsa_POLR2A_001 129 span n/a 47.29
PPIA NM_021130 hsa_PPIA_002 218 span n/a 50.5
RPL13 NM_000994 hsa_RPL13_001 81 span n/a 48.2
RPL32 NM_000994.3 & NM_033251 hsa_RPL32_001 152 span n/a 51.3
RPLP0 NM_001002 & NM_053275 hsa_RPLP0_001 88 flank 1088 span
SDHA NM_004168 hsa_SDHA_002 93 flank 1890 54.8
TBP NM_003194 hsa_TBP_002 87 flank 4707 57.5
TFRC NM_003234, NM_001128148 hsa_TFRC_001 188/td>

span n/a 43.6
TUBB NM_178014 hsa_TUBB_001 115 flank 1654 47.0
UBB NM_018955 hsa_UBB_001 86 span n/a 53.3
VDAC2C NM_001184783, NM_003375 hsa_VDAC2C_001 432/td>

span n/a 68.2
YWHAZ NM_003406, NM_145690, NM_001135699, NM_001135700, NM_001135701,& NM_001135702 hsa_YWHAZ_001 94 no n/a 45.1

 

    ? Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
    ? No secondary structure in the amplicon for any assay (positive delta G)
    ? Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
    ? Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
    ? Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation

standard tubesno bg