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£85 per single assay or five assays for £375
To order please send a Purchase Order to finance@qstandard.co.uk Free delivery within the UK |
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| Gene symbol | Accession number(s) | Product code | Amplicon length (bp) | Intron: span/flank | Intron length (bp) | Amplicon %GC |
|---|---|---|---|---|---|---|
| ACTB | NM_NM_205518 | gga_ACTB_001 | 145 | span | n/a | 59.3 |
| B2M | NM_001001750 | gga_B2M_001 | 157 | span | n/a | 59.9 |
| GAPDH | NM_204305 | gga_GAPDH_001 | 107 | span | n/a | 47.7 |
| PPIA | NM_001166326 | gga_PPIA_001 | 127 | span | n/a | 55.9 |
| YWHAZ | NM_001031343 | gga_YWHAZ_001 | 122 | span | n/a | 42.6 |
- ? Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
- ? No secondary structure in the amplicon for any assay (positive delta G)
- ? Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- ? Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- ? Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation
