eDNA positive controls and reference gene standards for RT-qPCR – amphibians

Reference gene standards – Xenopus

£85 per single assay or five assays for £375

To order please send a Purchase Order to finance@qstandard.co.uk

Free delivery within the UK


Gene symbol Accession number(s) Product code Amplicon length (bp) Intron: span/flank Intron length (bp) Amplicon %GC
ACTB NM_NM_205518 gga_ACTB_001 145 span n/a 59.3
B2M NM_001001750 gga_B2M_001 157 span n/a 59.9
GAPDH NM_204305 gga_GAPDH_001 107 span n/a 47.7
PPIA NM_001166326 gga_PPIA_001 127 span n/a 55.9
YWHAZ NM_001031343 gga_YWHAZ_001 122 span n/a 42.6


      – Specificity has been checked by melt peak analysis and the presence of only a single band on a gel


    – Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
    – Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation

eDNA positive control/qPCR standard – Great Crested Newt

This standard has been made to detect Great Crested Newt DNA using the Natural England assay.

£120 for sufficient standard to run 15 standard curves