Reference gene standards – Xenopus
£85 per single assay or five assays for £375
To order please send a Purchase Order to finance@qstandard.co.uk
Free delivery within the UK
| Gene symbol | Accession number(s) | Product code | Amplicon length (bp) | Intron: span/flank | Intron length (bp) | Amplicon %GC |
|---|---|---|---|---|---|---|
| ACTB | NM_NM_205518 | gga_ACTB_001 | 145 | span | n/a | 59.3 |
| B2M | NM_001001750 | gga_B2M_001 | 157 | span | n/a | 59.9 |
| GAPDH | NM_204305 | gga_GAPDH_001 | 107 | span | n/a | 47.7 |
| PPIA | NM_001166326 | gga_PPIA_001 | 127 | span | n/a | 55.9 |
| YWHAZ | NM_001031343 | gga_YWHAZ_001 | 122 | span | n/a | 42.6 |
- – Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- – Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- – Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation
eDNA positive control/qPCR standard – Great Crested Newt
This standard has been made to detect Great Crested Newt DNA using the Natural England assay.
£120 for sufficient standard to run 15 standard curves