| £85 per single assay or select any five assays for £375
To order please send a Purchase Order to finance@qstandard.co.uk Free delivery within the UK |
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Zebrafish
| Gene symbol | Accession number(s) | Product code | Amplicon length (bp) | Intron: span/flank | Intron length (bp) | Amplicon %GC |
|---|---|---|---|---|---|---|
| actb | NM_131031 | dre_actb_001 | 148 | span | n/a | 41.0 |
| eef1a1a | NM_200009 | dre_eef1a1a_001 |
169
|
span
|
n/a
|
51.5
|
| gapdh | NM_001115114 | dre_gapdh_001 | 85 | span | n/a | 54.1 |
| rpl13a | NM_212784 | dre_rpl13a_001 | 171 | span | n/a | 51.0 |
| tuba1 | NM_194388 | dre_tuba1_001 | 143 | flank | 1317 | 46.2 |
- ? Primers have been designed to avoid 3′ SNPs and secondary structure (self-annealing)
- ? No secondary structure in the amplicon for any assay (positive delta G)
- ? Specificity has been checked by melt peak analysis and the presence of only a single band on a gel
- ? Standard curves down to 10 copies per reaction have been run for each assay and all show excellent linearity (R² >0.99) Efficiency (>95%) and sensitivity (repeat of 10 copies consistently detected)
- ? Primers have been tested at an annealing temperature of 57°C. Higher annealing temperatures (up to 60°C) are possible, but require individual validation
